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Studies demonstrate that neutrophil-derived S100A8/A9 downregulates nuclear respiratory factor 1 (Nrf1) and suppresses expression of NADH dehydrogenase in mitochondrial complex I

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In vivo kinase assay 100 g of pUGW-CPK5-HA or pUGW-ILK5-HA(WT or S192A) constructs were introduced by polyethylene glycol (PEG)-mediated transformation into Arabidopsis protoplasts prepared from leaf tissues of three- to four-week-old plants described above, then incubated in a 21C growth chamber for 48 h under dark conditions with 2 mL W5 solution (2 mM MES pH 5.7, 154 mM NaCl, 125 mM CaCl 2 , and 5 mM KCl)

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