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Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining for apoptosis Apoptosis was also determined by the TUNEL assay using an in situ cell death detection kit conjugated with horse-radish peroxidase (POD) (Roche Applied Science, Indianapolis, IN, USA), according to the manufacturer's instructions

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If a sperm with fragmented DNA fertilizes an egg, it may lead to difficulties in the pregnancy progressing