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Description

The ligands for PD-1, namely PD-L1 and PD-L2 (members of the B7 family), are expressed on tumor cells and MDSCs [107], tumor-associated macrophages (TAMs), and M2 macrophages [22]

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After adding 1 ml of wash buffer (25 mM Tris, pH 7.5, 150 mM NaCl and 0.05% Tween-20), the beads were thoroughly resuspended, then collected again with the magnetic stand, and the supernatant was discarded