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To introduce the C-terminal Azo-tag into the coding region for the sfGFP reporter protein, two Gly codons and the amber stop codon were inserted between the C-terminal Strep -tag II and the final ochre stop codon, thus placing Pap as the last encoded residue in the reading frame, and this gene cassette was subcloned on pSB19 between the Xba I and Hin dIII restriction sites

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While it may still include the fluids mentioned above it can also include: Medications : Depending on the condition being treated, various medications are added to the IV drip to treat pain, nausea, infection, or other medical issues