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For differentiation, the hPSC aggregates were collected from the flask, transferred to a 50 ml conical tube and pelleted by centrifugation for 5 minutes at 300 x g at room temperature

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Using hydrogen peroxide-induced senescent TM3 Leydig cells as an in vitro model, the study confirmed that FOXO4 maintains senescent Leydig cell viability and suppresses apoptosis

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