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10.1016/S1097-2765(00)80356-1 Mol

25 l was transferred into the Nucleofector cassette and electroporated using the CA-189 protocol (Zou et al, 2021)

After the sample was applied on the skin surface of the donor part, the cell was incubated at 37C for 24 h

Researchers suggest that elevated gut pH, gut dysbiosis (i.e., a higher Firmicutes : Bacteroidetes ratio), obesity, or malabsorption might increase fecal SCFA, such as butyrate, which could have potential neurotoxic effects [25, 45, 46]