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Generation and quantification of LMW persulfides and polysulfides using GSH GSH (10 M) in 100 L 50 mM HEPES buffer (pH 7.4) was exposed to increasing concentration (010 M) of DTNB for 20 min and then reacted with Na 2 S or Na 2 S 2 (0 - 30 M) for 5 min