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The Utah Early Neuropathy Scale (UENS) focuses on small-fiber signs and pinprick testing, showing sensitivity of 78% and specificity of 85%, while the DN4 questionnaire targets neuropathic pain features with 83% sensitivity and 90% specificity, exceeding the NDS for painpredominant presentations (see Table 4)

The residual enzyme activity in liver homogenates of MCAD-KO mice, as measured with a clinical diagnostic assay for MCAD, was 9.3% of the wild-type activity (Additional file 2: Figure S2D)