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After washing the membrane with TBST buffer, incubate the membrane with secondary antibodies at room temperature on a shaker for 1.5 h

Clinically, the determination of serum lactate plays a very important role in guiding the treatment of patients with ischemic injury, especially in the treatment of tissue hypoxia and ischemia-reperfusion injury caused by cerebral ischemia and insufficient blood flow in patients with myocardial infarction 212,213,214,215