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Finally, blastocysts from each group were collected and incubated with 5 g/ml Hoechst 33342 solution at 38C for 5 min, followed by blastocysts mounting and microscope examination with cell number per blastocyst ( n = 20 for each group) further recorded

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It has been reported that EGLN2 (Egl-9 family hypoxia inducible factor 2) is a candidate driver of iron chelation-mediated inhibition of cell death, and the hydroxylase activity of EGLNs is iron-dependent[114, 115]