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Description

Structural basis for the active site inhibition mechanism of human kidney-type glutaminase (KGA)

10.7554/eLife.00762 163 RedisR

L., Dolled-Filhart, M

Glutamine dependency on oxygen consumption Briefly, 3.0 10 5 BT549 cells were seeded in a final volume of 100 L (complete RPMI media) in an XF24 plate (Agilent) and allowed to adhere for 1 h at 37 C in a 5% CO 2 humidified incubator, when more 150 L of same media was added and cells incubated in equal conditions for another 15 h