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Polyoxazoline-Based Gene Delivery System Mediated Tumor Vascular Normalization and Extracellular Matrix Degradation to Inhibit Cancer Growth, Recurrence, and Metastasis

The flowthrough was collected and flowed by gravity over Ni-NTA resin to remove HisULP1 and HisSmt3 proteins before concentration and gel filtration using an SD200 16/600 column in GF buffer