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2.2 Metabolic remodeling The occurrence and progression of PH are closely associated with multi-cellular metabolic reprogramming, which collectively drives the pathological process of the disease through abnormal switching of cellular energy metabolic pathways, regulation of key metabolites and signaling pathways, and bidirectional interactions between metabolic disorders and vascular remodeling (Figure 2)

The process was standardized as follows: At 37C during the whole night, 100 mg of adipose tissue was incubated in 700 L of an X-gal-containing staining solution

Then, samples were incubated with primary antibodies diluted in blocking solution at 4 C, overnight

August 16, 2009