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1 Introduction Autism Spectrum Disorder (ASD) is a complex neurodevelopmental condition characterized by deficits in social communication and the presence of restrictive and repetitive behaviors ( Although ASD is considered a common disorder, the exact etiology remains unknown ( The imbalance between neurotransmitters with excitatory and inhibitory properties is recognized as an etiological factor for ASD ( + K + 2Cl cotransporter 1 (NKCC1) and K + Cl cotransporter 2 (KCC2) were significantly correlated with GABAergic signaling ( Probiotics have the potential to reduce the symptoms of ASD by modulating neuroactive compounds

IVIG therapy, which involves infusions of antibodies derived from donor plasma, can help modulate the immune response and reduce inflammation, providing another valuable therapeutic option

Dietary Changes A diet rich in whole foods, lean proteins, healthy fats, and complex carbohydrates can support the HPA axis

Cell-free biology offers several advantages over living systems by bypassing cell growth and viability constraints, enabling customization of biosynthesis conditions for a single product, and offering unprecedented flexibility in designing and controlling biological systems