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The monocyte and macrophage annotations were not changed based on the myeloid subset

RNA-seq and analysis Total RNA isolation, cDNA library construction, and sequencing were performed at BGI (Beijing Genomics Institute, Shenzhen, Guangdong, China) using RNA-seq technology, as previously described 77,78

Standard dosing protocols: 100mg to 500mg NAD+ per injection, administered 2 to 3 times per week Testing Sensitivity: For first-time users, begin with a lower dose to evaluate tolerance

Pre-programmed settings make it easier to adjust the ice temperature depending on usage and can be applied for hockey, figure skating, and public skating