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Images were obtained at 20 magnification using a light microscope (Leica DM LB) and digitally photographed for automated image analysis

Oda Y, Tu C-L, Menendez A, Nguyen T, Bikle DD

The mobile phase consisted of solvent A (10 mM ammonium acetate with 1% acetic acid in water) and solvent B (acetonitrile) delivered at a flow rate of 0.35 mL/min with a linear gradient as follows: 25% B until 3 min, 2540% B over 3.9 min, 4070% B from 3.9 to 4 min, decreased to 25% B from 4 to 4.6 min, and maintained at 25% B until 5.5 min

In Conclusion MIC B12 injections offer a valuable tool for aesthetic professionals and weight loss clinics aiming to support patients through energy-boosting, mood-enhancing metabolic optimization