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Third, healthy mitochondria stabilize intracellular iron levels by participating in iron metabolism, reducing free iron-driven Fenton reactions and subsequent lipid radical chain damage, thereby suppressing ferroptosis at its origin

Targeting NOS1 either through Hsp90 inhibitors to block its mitochondrial localization or via specific inhibitors such as N-propyl-L-arginine may offer dual benefits: mitigating therapy resistance and addressing metabolic risk factors in CRC (57, 58)

Mitochondrial pyruvate carrier inhibition initiates metabolic crosstalk to stimulate branched chain amino acid catabolism

If the beads are in proximity, an energy transfer to the acceptor bead occurs, resulting in light production (luminescence), which is measured by a plate reader equipped for AlphaLISA signal detection