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At baseline, trained nurse interviewers administered a structured questionnaire to each subject to collect comprehensive information on demographics, occupation, lifestyle habits, medical history, usage of medication and dietary supplements, alcohol consumption, and past cigarette smoking history

At the end of the experiment (48 hours), 100 l were transferred to a cytometer tube, 0.5 l of 25 M Calcein-AM were added and, after 15 min of incubation at room temperature, 5 l of fluorescently labeled beads plus 195 l of PBS 1X were added and cells were analyzed by flow cytometry

(Activated charcoal is often used in healthcare facilities to treat food poisoning.) She also boosted her levels of glutathione, a potent antioxidant that supports detoxification

These compounds enter via ingestion, inhalation or dermal absorption and primarily accumulate in the GI tract (Hawkins et al., 2020)