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Grids were examined with a Hitachi 7600 or a Philips CM120 transmission electron microscope at 80 kV, and micrographs collected using AMT Image Capture Engine software controlling an AMT ER50 5-megapixel CCD camera (Advanced Microscopy Techniques Corp., Danvers, MA)

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Metformin enhances autophagy, reduces cardiac hypertrophy, and shows promise for improving functional outcomes in heart failure