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Glutathione IV therapy helps replenish these levels quickly and effectively by delivering the antioxidant directly into the bloodstream

Tissue dissociation and flow cytometry analysis Mouse skin samples were minced directly using a sharp blade and then incubated in Dulbeccos modified Eagles medium (DMEM) containing 1 mg/mL collagenase IV (900112-1, Gibco) and 12.5 kU DNase (D4263, Sigma) at 37 C for 90 min

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Cytosolic GSTs are encoded by at least 5 gene families (alpha, mu, pi, theta and zeta GST) whereas membrane-bound enzymes are encoded by single genes