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While ARG is able to catabolize arginine into ornithine and polyamines to promote cell proliferation and tissue repair, NOS breaks down arginine into NO to be involved in immune response and inflammation regulation ( in vivo during pathogen infection through three distinct mechanisms: 1) Arg1 depletes L-arginine from DCs, thereby preventing NOS2 from generating NO, which is effective in defending the organism against pathogen infections

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After treatment, 100 L of CCK-8 working solution was added to each well of the 96-well plate, followed by incubation at 37 C and 5% CO 2 in the dark for 1 h