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Thereafter, conditions and genes with similar profiles were comparatively analyzed using Hierarchical clustering tool with Euclidean distance method

In addition to these measures, it is important to keep well hydrated as the kidneys are the other major player in elimination and water ensures flow of these toxins into urine and out of the body

Analytical methods Grown cultures were removed from the incubator at a given time and biomass was measured as optical density at 600 nm (OD 600 ) and/or the dry cell weight (DCW)

Then, tissue was centrifuged and digested with Trypsin EDTA solution C to generate a single cell suspension, filtered through a 40 m cell strainer, and plated in DMEM/F12 with 2.5 mM L-glutamine, 12.5 mM D-glucose, 10% FBS, penicillin (50 U/ml), and streptomycin (50 g/ml), obtaining twelve independent cell lines (three for each treatment condition)