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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

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Unlike full-length growth hormone or growth hormone secretagogues, it provides lipolytic benefits without affecting IGF-1 levels, glucose metabolism, or growth-promoting effects

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