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Cell lines were cultured in the recommended medium and condition (DMEM medium with 10% FBS and 1% antibiotics (penicillin-streptomycin 10,000 U mL 1 ) at 37 C in a humidified atmosphere containing 5% CO 2 )

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Importantly, women appear to respond well at lower injectable doses, and the most common approach combines topical and injectable use simultaneously for maximum cosmetic effect