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Organotypic human skin culture models constructed with senescent fibroblasts show hallmarks of skin aging

doi: 10.1016/j.biortech.2020.123525 46 ZhangY.YeY.DingW.MaoX.LiY.GerkenH.et al

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Another region of equal scalp area affected by incipient alopecia in every subject was treated in the same way, using a lotion of equivalent composition but without the Oleuropein (active preparation 2): Ethanol 48.0% (v/v) Water 28.2% (v/v) Propanediol 20.0% (v/v) Ethoxy-glycol 1.5% (v/v) Sodium bicarbonate 0.5% (w/v) Glutathione / Vitamin F thioester 0.4% (w/v) Lactic acid 0.2% (w/v) Methyl nicotinate 0.05% (w/v) Another region of equal scalp area affected by incipient alopecia in every subject was treated in the same way, using a lotion of equivalent composition, but without the Glutathione /Vitamin F thioester (active compound 3): Ethanol 48.0% (v/v) Water 28.2% (v/v) Propanediol 20.0% (v/v) Ethoxy-glycol 1.5% (v/v) Oleuropein (from dry extract of olive leaf) 1.2% (w/v) Sodium bicarbonate 0.5% (w/v) Lactic acid 0.2% (w/v) Methyl nicotinate 0.05% (w/v) Another region of equal scalp area affected by incipient alopecia in every subject was treated in the same way, using a lotion of equivalent composition, but without the Glutathione/Vitamin F thioester (control preparation 1): Ethanol 49.0% (v/v) Water 28.2% (v/v) Propanediol 20.0% (v/v) Ethoxy-glycol 1.5% (v/v) Sodium bicarbonate 0.5% (w/v) Lactic acid 0.2% (w/v) Methyl nicotinate 0.05% (w/v) At the end of twenty-one days (Checkpoint 1) and forty -two days (Checkpoint 2) of treatment being performed, the trichological examination was performed using photo- tricogrammetry on 2 cm 2 randomly chosen treated areas and control areas each