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10.3390/ijms21010243 [DOI] [PMC free article] [PubMed] [Google Scholar] 42.Takenaka K, Fujii T, Kawamoto A, Suzuki K, Shimizu H, Maeyashiki C, Yamaji O, Motobayashi M, Igarashi A, Hanazawa R, Hibiya S

doi: 10.1002/hup.829

Activation of GPX4 in a Cell-Free Assay By coupling the oxidation of NADPH to NADP + by oxidized glutathione in the presence of glutathione reductase, GPX4 activity was assessed by measuring the decrease in NADPH fluorescence emission at 460 nm (excitation at 335 nm)