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Choosing the right diluent is more than a minor detailit directly impacts the stability and sterility of your final solution

We next tested this hypothesis

3) The general procedure for studying the effect of the pH on the rate of SetCys to Cys conversion is illustrated with the following conditions: 1 mM peptide, 200 mM MPAA, 100 mM TCEP, 100 mM ascorbate, pH 6.90 in 0.1 M phosphate buffer