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Because it is essentially water without sufficient solute, direct intravenous administration can cause red blood cell lysis (hemolysis) due to its hypotonic nature

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While lipo B12 injections are very safe for most people, its best to consult a doctor before starting injections, especially if you: Have a personal or family history of autoimmune disorders Have methylmalonic acidemia or other B12-related genetic disorders Are you pregnant or breastfeeding Have kidney disease or liver disease Have leukaemia or other blood disorders Have had allergic reactions to B12 supplements before In these cases, your doctor can determine if injections are suitable or an alternative treatment is better

With the application of the surrogate analyte approach, the LOD and LOQ were calculated from [ 15 N 13 ]-CNCbl instead of CNCbl based on the reverse LC-MS/MS response curve (details in the LOD and LOQ section)