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The trial objectives were to demonstrate the benefit of darbepoetin alfa treatment of the anemia to a target hemoglobin level of 13 g/dL, when compared to a "placebo" group, by reducing the occurrence of either of two primary endpoints: (1) a composite cardiovascular endpoint of all-cause mortality or a specified cardiovascular event (myocardial ischemia, CHF, MI, and CVA) or (2) a composite renal endpoint of all-cause mortality or progression to end stage renal disease

The Rubisco bands were stained with Ponceau S (Merck Sharp & Dohme Corp., NJ, USA) as a loading control

The basis for the hydroxyl radical's extreme reactivity in lipid systems is that at very low concentrations it initiates a chain reaction involving triplet oxygen, the most abundant form of oxygen in the cell (Benderitter et al., 2003)

Therefore, owing to its combined analgesic and pro-healing activities, neurotensin holds a strong potential for incorporation into topical peptide-based therapies targeting chronic wounds