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General categories of these cytotoxic payloads include anti-microtubule agents (i.e., DM1) and agents that exert DNA damage such as topoisomerase I inhibitors (i.e., SN-38, deruxtecan) [16]

Glud1-Flag immunoprecipitation YUMM3.3SelO cells co-expressing Glud1-Flag or Glud1-Flag and SelO or SelO D338A were resuspended in lysis buffer containing 50 mM Tris pH 8, 150 mM NaCl, 1% TX-100, 1X Roche cOmplete protease inhibitor, 1 mM EDTA, and nutated at 4 C for 20 min

Cell lines stably expressing cytosolic GFP and surface LaccID were achieved by lentivirus infection, followed by FACS sorting

For patients with melasma or deeper skin tones, mandelic acid and low-concentration glycolic peels are preferred over aggressive protocols to minimize post-inflammatory risk