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To separate PM 2.5 and solvent, 50 mL of the supernatant was centrifuged at 3220 x g for 5 min (Centrifuge 5810, Eppendorf, Hamburg, Germany)

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[Emphasis added.]48 From the late 1970s through the 1980s RJ Reynolds' research consistently tracked menthol smoking trends by race, gender and age and saw the increasing popularity of menthols among the young, women and African Americans.44 4951 By 1978 Philip Morris, which did not have a strong menthol product, was concerned about this trend34: We knew that Blacks, females, and younger smokers were more likely to smoke menthol cigarettes than whites, males, and older smokers