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Caloric restriction inhibits mammary tumorigenesis in MMTV-ErbB2 transgenic mice through the suppression of ER and ErbB2 pathways and inhibition of epithelial cell stemness in premalignant mammary tissues

A key objective for Lefkovits with the experiment was to get non-responding cultures as this was key to being able to calculate the number of precursor cells involved in antibody formation using the Poisson distribution equation (Lefkovits 2025)

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