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From the resected liver tissue, adjacent noncancerous healthy tissue was collected, minced and enzymatically digested with collagenase VI (Sigma-Aldrich) to obtain a single cell suspension

M.SinghH.DarR

Aygun C, Kocaman O, Sahin T, et al

Thus, studies conducted on animals have demonstrated that inhibition of the endogenous methylation demand by creatine supplementation can reduce Hcy levels, but these results have not been reproduced in human subjects