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In the absence of consensus among physicians, patients are subject to conflicting advice, causing uncertainty and distress

31 In another study conducted in Brazil, using NAC as adjunctive therapy at a dose of 600 mg orally twice a day for eight weeks, showed that 61.1% of patients with NAC had negative smear tests compared to 33.3% in the control group

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Isolation of polar metabolites For the metabolomics of tissues, 100200 mg of weighed tissue was homogenized in 1 ml of ice-cold 80% methanol with a QIAGEN Tissue Lyser II (three sets of 2 min on maximum speed, with 2 min of rest on dry ice in between sets)