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The 1 ml assay mixture contained potassium phosphate buffer (50 mM, pH 7.0), 2 mM EDTA, 0.2 mM NADPH, 0.5 mM GSSG, and leaf extract

After separation and hydrolysis or extraction, the protein hydrolysate, bioactive peptides, and especially thrombin, fibrinogen, heme iron peptide, and globin, can be obtained (Figure 3)

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These observations challenge purely microbe-centric models, suggesting ENS activity may constrain microbial niches via nutrient fluxes, redox shifts, and resource partitioning dynamics amplified during exercise