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perfoliata Omega class GST sequences clustering in previous phylogenetic analyses were aligned separately using ClustalW multiple sequence alignment and used for the generation of an Omega specific phylogenetic tree as described above

use within approximately 30 days [7]

The relative expression of target genes was calculated using the 2e(-Ct) method ( ELISA To access the intracellular protein levels of CCL3 and IL-1B, cell precipitates of aliquots from endpoints A and B were homogenized in 1X PBS, diluted in Milli-Q H 2 O 1:2 and the cells disrupted by mechanical lysis in the TissueLyser (Qiagen) for 2 minutes at 30 Hz and three freeze/thaw cycles (20 to -20C)

Glutathione provides a fascinating fail-safe mechanism known as protein S-glutathionylation