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32 The positive association between frequency of MTHFR -C677T and MTHFR -A1298C polymorphisms and latitude documented in the current study suggests that in areas closer to the equator, which are increasingly exposed to UVR, activity of the MTHFR enzyme reflects greater functional competency because of the absence of the MTHFR polymorphisms

Clinic Information [Clinic Name] Biotope Clinic [Location] Tokyo, Japan [Nearest Station/ Nearest Tourist Destination] Located in Minato-Ku, Biotope clinic is easily accessible via train and is only 1-min walk from Sirokanedai Station Exit 1 [Clinic Opening Time] Monday - Saturday (10:00AM - 1:00PM, 2:00PM - 7:00PM)

Cell Counting Kit-8 (CCK-8) Assay for Cell Viability and Cell Proliferation Cell viability was determined using the CCK-8 solution (Beyotime, China), under the manufacturers instructions

The exact reasons and mechanisms regarding the failure of these studies to produce the expected beneficial effects remain largely unknown