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Tumors were snap-frozen in acetone/dry ice mixture and sectioned (5 m) for blood vessels (anti-CD31 (Abcam, ab28364, 1:50) + AF488 secondary (Abcam, ab150073, 1:400 dilution)) and cellular nuclei (DAPI) staining, and then visualized by confocal microscopy (Zeiss LSM880, v.14.022.021)

A pharmacokinetics study published in Frontiers in Pharmacology found an average intravenous elimination half-life (t1/2) of 15.2 minutes in rats, with the intramuscular peak plasma concentration reached at 3 minutes post-injection

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This method bypasses the digestive system, ensuring maximum absorption and effectiveness