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These virus-induced vesicles could damage mitochondrial membrane integrity, release mt-DNA into circulation, compromise innate immunity, and trigger an exacerbated pro-inflammatory response in COVID-19 patients 164

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This constraint may reduce experimental throughput and necessitate optimization strategies to maximize the use of scarce patient-derived material

Two days later, supernatants were harvested from these passage 1 cultures, and a 100 l aliquot of the cleared supernatant was incubated with the same concentration of antibodies and then used to infect 5 10 5 293 T/hACE2cl.22 cells in 6-well plates, as before