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C, deletion of amino acids 430559

We also used alkaline phosphatase, a disulfide-dependent enzyme that had been engineered not to be secreted, to detect thiol oxidation events inside the cytoplasm

I know how to do control experiments

Glutathione is a key antioxidant and detoxifier for the body because glutathione protects cells from the damage of oxidative stress by eliminating free radicals and heavy metals