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The reduction of roGFP2(S 2 ) is therefore a good example for an enzyme using an alternative reaction pathway to overcome a kinetic challenge, and we hypothesize that other non-glutathione disulfide substrates with reactive proximal cysteinyl residues and rather negative redox potentials also require a ternary complex for their reduction

Surgical procedure The rats were tracheotomized, and a heparinized catheter was inserted into the femoral artery to record systolic (SBP) and diastolic (DBP) BP