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The flow rate was kept constant at 0.2 mL/min and the gradient was programmed as follows: from a starting point of a 72:28 ratio of A:B, the gradient moved to 71.2% B over 12 min, then moved to 100% B over 1 min, remained at 100% B for 3 min, then moved back to the starting ratio of 72:28 A:B (72:28) over 0.5 min, and remained at this ratio for 3 min

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"Abstract PRO 2: LEE01 1 : an orally bioavailable, selective small molecule inhibitor of CDK4/6 reactivating Rb in cancer." Mot Cancer Ther 2013, 12, 1 1 S:PR02