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The desensitisation procedure should be started by the 1/1001/1000 solutions of the drug target or the 1/101/1000 solutions of positive intradermal test dose and in increasing doses by doubling with 3090min intervals until the therapeutic dose is achieved.5 Desensitisation must be carried out in a hospital setting by experienced personnel with adequate resuscitation equipment readily available

The standard western blot protocol was performed as our previous study 6

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It determines how much volume you need for a specific dose