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In the mid-to-late 1960s, furosemide was synthetized and quickly gained popularity due to its strong diuretic effects, fast action and effectiveness, especially in treating heart failure, hypertension, edema and kidney failure (Ingram, 1964

The route is determined by the clinical goal: injectable for systemic or musculoskeletal use, oral for gut-specific applications

Biochim Biophys Acta 1989

To construct an oxidative stress IEC-6 cell model using HO, cells were seeded in 96-well plates and exposed to HO at concentrations of 100, 200, 300, 400, 500, 600, and 700 mol/L