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3.2.2 Subacute oral toxicity study 3.2.2.1 Clinical observations Subacute oral toxicity study on SG was performed for 28-day in a graded range of doses: 500 mg/kg, 1,000 mg/kg and 2000 mg/kg body weight

Discard the myelin in the supernatant and harvest the cellcontaining fraction in an appropriate medium (see refs 12-16)

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After Blush regularization, a focus mask that covers the SLC33A1 MAP density but excludes the detergent micelle density, which we refer to as an SLC33A1 MAP TMD focus mask was used to for local refinement of the transmembrane domain, yielding a construction at a resolution of 3.26