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doi: 10.1210/endocr/bqab161 98 DuboisSLAcosta-MartnezMDeJosephMRWolfeARadovickSBoehmUet al

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Yeast cells were incubated with diamide or DTT, then precipitated by treatment with trichloroacetic acid (TCA), and then free thiols (i.e., reduced cysteines) were modified with the alkylating agent 4acetamido4maleimidylstilbene2,2disulfonic acid (AMS)