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This effect could be associated with the antioxidative properties of the supplementation, which may attenuate the ROS-driven positive feedback loop that otherwise sustains Cyp2e1 induction ( Cyp2e1 gene expression through methylation at the genes promoter region, representing an potential alternative mechanism of regulation ( Compromised intestinal permeability following ethanol exposure facilitates the leakage of gut bound bacterial endotoxin, lipopolysaccharide (LPS) into the circulation ( Kupffer cells resulting in massive secretion of the TNF-, a pro-inflammatory cytokine that initiates a vicious cycle of inflammation by coordinating the immune responses (Figures 13A,B)

The freeze-drying process preserves the blue copper peptide without water to ensure that it reduces the possibility of bacterial contamination caused by moisture, and also avoids the situation where multiple components "fight" with the blue copper peptide

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Kitada S, Pedersen IM, Schimmer AD, Reed JC