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10.3390/ijms241612948 191 WuH.WangJ.JiangH.LiuX.SunX.ChenY.et al (2022)

It involves using a device with fine needles to create tiny punctures in the skin, which stimulates collagen production and helps smooth out the skins surface

Additionally, the absence of standardized protocols that limit reproducibility and the difficulty of monitoring cells within the 3D culture further contribute to the limited availability of organoid cultures [180]