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Seahorse-based oxidative phenotyping The oxygen consumption rates (OCR) and the extracellular acidification rates (ECAR) of cell lines were quantified using the Seahorse Extracellular Flux Analyzer (XFe96, Agilent)

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For anti CD31 staining, slices underwent antigen retrieval by boiling in Citrate Buffer (pH 6) at 95 C for 10 min

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However, many of the experimental drugs were discontinued in clinical trials due to side effects (Phillips, 2020)

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