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Both PM 2.5 and smoking have been reported to promote inflammation and cell death in the lungs [11,12,13,14,15,16]

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This step and the next step of Western blotting were necessary, because the proteins evaluated in the Kinex TM KAM 1.0 antibody microarrays were not denatured, which increases the opportunity for false positives and false negatives due to antibody cross-reactivity, protein-protein interactions, and blocked epitopes in protein complexes